| Solution Information | help | |
| Enzyme: | Bcl-2-like protein 1 | |
| inhibitor: | BDBM61873 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this biochemical assay is to determine dose response curves for compounds identified as active in a set of previous experiments entitled, "Primary biochemical high throughput screening assay to identify inhibitors of BCL2-related protein, long isoform (BCLXL)" (AID 2129), and that confirmed activity in a set of experiments entitled, "Counterscreen for MCL1 inhibitors: fluorescence polarization-based biochemical high throughput confirmation assay for inhibitors of BCL2-related protein, long isoform (BCLXL)" (AID 2166). This assay also serves as a counterscreen for a set of previous experiments entitled, "Fluorescence polarization-based primary biochemical high throughput screening assay to identify inhibitors of myeloid cell leukemia sequence 1 (MCL1) interactions with BIM-BH3 peptide." (AID 2057) and confirms BH3 mimetic activity of compounds of interest. This fluorescence polarization (FP)-based assay monitors binding of the BH3 domain of the Bcl-2 fam | |
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